E-SMDB-1613 - Transcription profiling to analyse the effect of the date of preparation of human polyA RNA
Released on 6 June 2006, last updated on 27 March 2012
This experiment was designed to assess the reproducibility of microarray hybridization using poly(A)+RNA. BC2 total RNA was isolated from which poly(A)+RNA was subsequently isolated. Three arrays were hybridized on the same day using arrays from the same batch. Two were done three months later using poly(A)+RNA freshly isolated from the same total RNA.
transcription profiling by array, replicate
Optimization and evaluation of T7 based RNA linear amplification protocols for cDNA microarray analysis. Zhao H, Hastie T, Whitfield ML, Borresen-Dale AL, Jeffrey SS.