E-SGRP-2 - Transcription profiling of Schistosoma mansoni lung schistosomulum
Submitted on 16 November 2005, released on 20 November 2005, last updated on 2 May 2014
The lung schistosomulum of Schistosoma mansoni is a validated target of protective immunity elicited in vaccinated mice. To identify genes expressed at this stage we constructed a microarray, representing 3088 contigs and singlets, with cDNA derived from in vitro cultured larvae, and used it to screen RNA from seven life cycle stages. Clustering of genes by expression profile across the life cycle revealed a number of membrane, membrane-associated and secreted proteins up-regulated at the lung stage, that may represent potential immune targets. Two promising secreted molecules have homlogy to antigens with vaccine and/or immunomodulatory potential in other helminths.
transcription profiling by array, innate behavior