E-NASC-9 - Transcription profiling by array of A. thaliana hog-1 and wild type whole plants to investigate downstream targets of DNA methylation

Status
Released on 30 March 2004, last updated on 25 October 2012
Organism
Arabidopsis thaliana
Samples (4)
Array (1)
Protocols (15)
Description
The C-line contains additional copies of chalcone synthase (CHS) and these result in silencing of the endogenous copy at tt4. The clv1-2 mutation was introduced into this line as a second marker. The line was mutagenised and several revertants obtained which show reduced silencing. The best characterised of these is hog1. The hog1 mutant shows reduced DNA methylation and more intact CHS transcript in Northern blots. I have been funded by the BBSRC gene flow initiative to look for downstream targets of DNA methylation by looking for genes which are up or down regulated by hog1. Three week old plants of the genotype C/C clv1-2 with or without the hog1 mutation will be used to prepare RNA for the study. I have set the sample size at two slides for each sample.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-NASC-9.idf.txt
Sample and data relationshipE-NASC-9.sdrf.txt
Raw data (1)E-NASC-9.raw.1.zip
Processed data (1)E-NASC-9.processed.1.zip
Array designA-AFFY-16.adf.txt
Links