E-NASC-42 - Transcription profiling of Arabidopsis irradated with cFR (far-red) light for 18 hr and treated with 20 nM of flumioxazin

Released on 12 January 2005, last updated on 2 May 2014
Arabidopsis thaliana
Samples (6)
Array (1)
Protocols (7)
Plastids communicate with the nucleus by means of retrograde plastid signals. The far-red (FR) light insensitive Arabidopsis mutant laf6 disrupted in a plastid-localised ABC-like protein (atABC1) accumulates the plastid signal protoporphyrin IX (proto IX) and has attenuated nuclear gene expression (Moller et al.2001 Genes Dev. 15:90-103). Our data suggests that proto IX accumulation results in hypocotyl elongation in response to FR light and we have demonstrated that by inhibiting the plastid localised protoporphyrinogen IX oxidase (PPO) using flumioxazin wild-type plants phenocopy laf6 by accumulating proto IX with a concomitant loss of hypocotyl growth inhibition in a dose-dependent manner. It is at present unclear what effect increased proto IX has on nuclear gene expression and how this is integrated with photomorphogenic responses such as hypocotyl elongation.
Experiment types
transcription profiling by array, compound treatment, individual genetic characteristics, strain or line
Investigation descriptionE-NASC-42.idf.txt
Sample and data relationshipE-NASC-42.sdrf.txt
Raw data (1)E-NASC-42.raw.1.zip
Processed data (1)E-NASC-42.processed.1.zip
Array designA-AFFY-16.adf.txt
R ExpressionSetE-NASC-42.eSet.r