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E-MTAB-9923 - Total RNA-seq in the A549 cell line after glucocorticoid re-induction
Last updated on 16 December 2020, released on 3 May 2021
We performed total RNA-seq in the A549 cell line. Cells were treated with either 100 nM dexamethasone or 0.1% ethanol. After 4 hours, cells were washed 2x with PBS and cultured in hormone-free medium for 24 hours, after which cells were treated again for 4 hours with either 100 nM dexamethasone or 0.1% ethanol.
RNA-seq of total RNA, compound treatment design
Glucocorticoid receptor activation induces gene-specific transcriptional memory and universally reversible changes in chromatin accessibility. Melissa Bothe, René Buschow, Sebastiaan H. Meijsing.