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E-MTAB-9915 - Circularized chromosome conformation capture (4C-seq) in A549 cells after glucocorticoid re-induction
Last updated on 15 December 2020, released on 3 May 2021
We performed 4C-seq in the A549 cell line to investigate changes in chromatin interactions at the ZBTB16 locus. Cells were treated with either 100 nM dexamethasone or 0.1% ethanol. After 4 hours, cells were washed 2x with PBS and cultured in hormone-free medium for 24 hours, after which cells were treated again for 4 hours with either 100 nM dexamethasone or 0.1% ethanol. Experiments were performed in two biological replicates. For each treatment, 4C-seq data were generated for the following two viewpoints: ZBTB16 promoter-proximal viewpoint coordinates: chr11:113929670-113930464 (hg19) ZBTB16 intronic viewpoint coordinates: chr11:114051224-114051804 (hg19)
4C, compound treatment design
Glucocorticoid receptor activation induces gene-specific transcriptional memory and universally reversible changes in chromatin accessibility. Melissa Bothe, René Buschow, Sebastiaan H. Meijsing.