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E-MTAB-9914 - Glucocorticoid receptor profiling (ChIP-seq) in U2OS-GR cells 24 hours after hormone washout
Last updated on 15 December 2020, released on 3 May 2021
We performed ChIP-seq targeting the glucocorticoid receptor (GR) in the U2OS-GR cell line. The cell line is derived from U2OS ATTC:HTB-96 and stably transfected with an expression construct for rat GR. The cells were treated with 100 nM dexamethasone for 4 hours, washed 2x with PBS and cultured in hormone-free medium for 24 hours before harvest.
Glucocorticoid receptor activation induces gene-specific transcriptional memory and universally reversible changes in chromatin accessibility. Melissa Bothe, René Buschow, Sebastiaan H. Meijsing.