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E-MTAB-9909 - Studying changes in chromatin accessibility in U2OS-GR cells by ATAC-seq directly after 4-hour glucocorticoid treatment and following a 24-hour hormone washout
Last updated on 14 December 2020, released on 3 May 2021
We performed ATAC-seq in the U2OS-GR cell line. The cell line is derived from U2OS ATTC:HTB-96 and stably transfected with an expression construct for rat GR. Cells were treated with dexamethasone (100 nM) or vehicle (ethanol) for 4 hours and either harvested immediately or washed 2x with PBS and subsequently cultured in hormone-free medium for 24 hours before harvest.
ATAC-seq, compound treatment design
Glucocorticoid receptor activation induces gene-specific transcriptional memory and universally reversible changes in chromatin accessibility. Melissa Bothe, René Buschow, Sebastiaan H. Meijsing.