E-MTAB-986 - ChIP-seq study using a cell line model of ER_ AR+ molecular apocrine tumours (AR_FoxA1_molecular_apocrine)

Status
Released on 18 February 2012, last updated on 2 May 2014
Organism
Homo sapiens
Samples (3)
Protocols (4)
Description
Breast cancer is a heterogeneous disease and several distinct subtypes exist based on differential gene expression patterns. Molecular apocrine tumours were recently identified as an additional subgroup, characterised as oestrogen receptor negative and androgen receptor positive (ER_ AR+), but with an expression profile resembling ER+ luminal breast cancer. One possible explanation for the apparent incongruity is that ER gene expression programmes could be recapitulated by AR. Using a cell line model of ER_ AR+ molecular apocrine tumours (termed MDA-MB-453 cells), we map global AR binding events and find a binding profile that is similar to ER binding in breast cancer cells. We find that AR binding is a near-perfect subset of FoxA1 binding regions, a level of concordance never previously seen with a nuclear receptor. AR functionality is dependent on FoxA1, since silencing of FoxA1 inhibits AR binding, expression of the majority of the molecular apocrine gene signature and growth cell growth. These findings show that AR binds and regulates ER cis-regulatory elements in molecular apocrine tumours, resulting in a transcriptional programme reminiscent of ER-mediated transcription in luminal breast cancers.
Experiment types
ChIP-seq, cell type comparison, disease state, in vitro, replicate
Contact
Citation
Androgen receptor driven transcription in molecular apocrine breast cancer is mediated by FoxA1. Robinson JL; Macarthur S; Ross-Innes CS; Tilley WD; Neal DE; Mills IG; Carroll JS.
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-986.idf.txt
Sample and data relationshipE-MTAB-986.sdrf.txt
Processed data (1)E-MTAB-986.processed.1.zip
Links