E-MTAB-970 - Transcription profiling by high throughput sequencing of Sox17.Epi and Endo cells from mouse embryos
Released on 20 July 2012, last updated on 3 June 2016
This experiment has been done to understand the dynamics of gene expression in Sox17.Epi and Endo cells using multiplex RNA-Seq technology. Sox17.Epi (Sox17^GFP+EpCAM+) and Sox17.Endo (Sox17^GFP+EpCAM-) cells were isolated by FACS from E9.5 mouse embryos. The spatial comparison between Sox17.Epi versus Sox17.Endo provides insight into which genes are differentially of specifically expressed in two populations.
RNA-seq of coding RNA, cell type comparison design, transcription profiling by high throughput sequencing
Dual lineage-specific expression of Sox17 during mouse embryogenesis. Choi E, Kraus MR, Lemaire LA, Yoshimoto M, Vemula S, Potter LA, Manduchi E, Stoeckert CJ Jr, Grapin-Botton A, Magnuson MA. , PMID:22865702