24 protocols
AccessionType
nucleic acid labeling protocol
Labeling of samples was carried out according to the manufacturer’s standard protocol
labeling
Ambion MessageAmp Biotin II Enhanced
nucleic_acid_extraction
Liver Biopsy Tissue was frozen in RNALater. RNA was extracted using Trizol and purified on RNeasy Columns
labeling
Preparation of biotinylated cRNA was performed according to the standard (one-cycle) Affymetrix U133A 2.0 sample preparation protocol, starting with 1ug of total RNA
nucleic_acid_extraction
Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
nucleic_acid_extraction
Title: Trizol extraction. Description:
labeling
Affymetrix protocol
nucleic_acid_extraction
trizol
nucleic_acid_extraction
Total RNA was extract by Trizol
labeling
In vitro transcription was performed to obtain biotin labeled cRNA according to Affymetrix protocols described at http://www.affymetrix.com/support/technical/manuals.affx
labeling
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
nucleic_acid_extraction
Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by phenol/chloroform purification using PLG tubes.
labeling
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
nucleic_acid_extraction
Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by phenol/chloroform purification using PLG tubes.
specified_biomaterial_action
Liver tissues were collected from patients undergoing a PHx surgery (1.5, 42 and 81 years), and snap-frozen in liquid nitrogen and then stored at –80°C until analysis.
labeling
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
nucleic_acid_extraction
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
labeling
Approximately 5 Âμg of total RNA was processed to produce biotinylated cRNA targets.
nucleic_acid_extraction
Total RNA extracted using standard Trizol protocol, followed by Qiagen RNeasy column clean-up
labeling
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms total RNA.
nucleic_acid_extraction
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
bioassay_data_transformation
Samples were normalised using RMA from R Bioconductor.
grow
Fresh-frozen liver samples were collected from normal liver (n=4) and both background liver (n=7) and HCC (n=7) from patients with haemochromatosis (HH) undergoing liver transplantation or resection for HCC. Normal liver samples were harvested during liver resection for colorectal cancer metastasis from patients who had received systemic chemotherapy some time prior to the operation.
nucleic_acid_extraction
Total RNA was extracted from frozen tissues using the phenol-chloroform method. Samples were then DNase treated (Turbo DNase, Ambion) followed by purification of RNA molecules >200bp with RNeasy mini spin columns (Qiagen).