E-MTAB-9241 - bulk RNA-seq of Anopheles gambiae hemocytes, guts, and carcasses after blood-feeding, Plasmodium berghei infection, or sugar feeding
Last updated on 13 August 2020, released on 27 August 2020
Anopheline mosquitoes transmit Plasmodium parasites to humans, and are responsible for an estimated 219 million cases of malaria, leading to over 400,000 deaths annually. The mosquito’s immune system limits Plasmodium infection in several ways, and hemocytes, the insect white blood cells, are key players in these defense responses. However, the full functional diversity of mosquito hemocytes and their developmental trajectories have not been established. We use bulk RNA sequencing (scRNA-seq) to analyze the transcriptional profiles of hemocytes, of guts, and of carcasses of mosquito hemocytes in response to blood feeding or infection with Plasmodium. Data from three independent biological replicates for each condition and time-point (day 0, 1, 2, 3, and 7 after sugar-feeding, blood-feeding or P. berghei infection).
RNA-seq of coding RNA, organism part comparison design, stimulus or stress design, time series design
Mosquito cellular immunity at single-cell resolution. Gianmarco Raddi, Ana Beatriz F Barletta, Mirjana Efremova, Jose Luis Ramirez, Rafael Cantera, Sarah A. Teichmann, Carolina Barillas-Mury, Oliver Billker.