E-MTAB-921 - Transcription profiling of mouse E10.5 female embryos wild-type and mutant for Smchd1
Released on 1 April 2012, last updated on 26 October 2015
To investigate the role of the Smchd1 protein in epigenetic silencing of autosomal loci, we carried out a comparative microarray gene expression analysis of wild-type and Smchd1-/- female embryos at E10.5. Embryos were collected at E10.5 after natural mating, sexed and genotyped. Total RNAs were extracted from three individual wild-type and three Smchd1-/- female embryos and cRNAs prepared from these samples was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 array.
transcription profiling by array, co-expression, in vivo, replicate
Epigenetic functions of smchd1 repress gene clusters on the inactive X chromosome and on autosomes. Gendrel AV, Tang YA, Suzuki M, Godwin J, Nesterova TB, Greally JM, Heard E, Brockdorff N. , Europe PMC 23754746