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E-MTAB-8142 - Single cell RNA-seq of human breast skin cells

Status
Released on 10 February 2021, last updated on 11 July 2021
Organism
Homo sapiens
Samples (96)
Protocols (5)
Description
Cells were generated for a single cell atlas of adult human skin to dissect the cellular and molecular organisation underpinning human skin immune barrier function. Surplus skin from breast reconstruction surgery was collected (n=3), then the top 200 μm layer was taken. Epidermis was separated from the dermis, and both layers were separately digested. Single cells were FACS sorted into eight categories, then loaded onto a 10x Genomics Chromium Controller, and sequenced on an Illumina HiSeq 4000.
Experiment type
RNA-seq of coding RNA from single cells 
Contacts
Citation
Developmental cell programs are co-opted in inflammatory skin disease. Reynolds G, Vegh P, Fletcher J, Poyner EFM, Stephenson E, Goh I, Botting RA, Huang N, Olabi B, Dubois A, Dixon D, Green K, Maunder D, Engelbert J, Efremova M, Polański K, Jardine L, Jones C, Ness T, Horsfall D, McGrath J, Carey C, Popescu DM, Webb S, Wang XN, Sayer B, Park JE, Negri VA, Belokhvostova D, Lynch MD, McDonald D, Filby A, Hagai T, Meyer KB, Husain A, Coxhead J, Vento-Tormo R, Behjati S, Lisgo S, Villani AC, Bacardit J, Jones PH, O'Toole EA, Ogg GS, Rajan N, Reynolds NJ, Teichmann SA, Watt FM, Haniffa M. , PMID:33479125
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-8142.idf.txt
Sample and data relationshipE-MTAB-8142.sdrf.txt
Processed data (2)E-MTAB-8142.processed.1.zip, E-MTAB-8142.processed.2.zip
Additional data (1)E-MTAB-8142.additional.1.zip
Links