E-MTAB-801 - Genotype mixing affects gene expression associated with hygienic behavior
Released on 24 September 2012, last updated on 2 May 2014
Analysis used total RNA samples of individual brains of low hygienic strain worker bees that were derived from experimental colonies combined of low hygienic genotypes (L pure colonies) resp. low and high hygienic genotypes (L/H mixed colonies). Four-condition experiment: individuals that did perform hygienic behavior (uncapping) vs individuals that did not perform hygienic behavior (non-hygienic); individuals from L pure colonies vs individuals from L/H mixed colonies. A total of 76 biological replicates was analyzed, subdivided into 24 individuals that did perform hygienic behavior in mixed colonies; 24 individuals that did perform hygienic behavior in pure colonies; 14 individuals that did not perform hygienic behavior in mixed colonies; 14 individuals that did not perform hygienic behavior in pure colonies. Each individual brain RNA sample was divided into two subsamples labeled with cy3 resp. cy5. Loop Design was applied so that each individual brain sample was directly compared to at least two other samples.
transcription profiling by array, co-expression, dye swap, environmental history, innate behavior, loop, replicate, strain or line
Mixing of honeybees with different genotypes affects individual worker behavior and transcription of genes in the neuronal substrate. Gempe T, Stach S, Bienefeld K, Beye M. PLoS One 7(2):e31653 (2012), Europe PMC 22348118