E-MTAB-8005 - RNA-Seq of murine trisomic B cell precursors expressing KRAS_G12D
Submitted on 14 June 2018, last updated on 30 May 2019, released on 9 June 2019
To better understand the molecular bases of the oncogenic cooperation between trisomy 21 and RAS/MAPK pathway activation, KRASG12D was overexpressed in wild-type (WT) or partially trisomic (Ts1Rhr) bone marrow cells. Transduced mCherry-positive murine B cell progenitors were platted in methylcellulose to form B cell precursors colonies (CFU-preB). After a week, B220+/CD19+/CD25- B cells were harvested to perform RNA-sequencing experiments.
RNA-seq of coding RNA, genetic modification design, genotype design