E-MTAB-7922 - Plasticity of Mitochondrial DNA Inheritance and its Impact on Nuclear Gene Transcription in Yeast Hybrids grown on YP Glycerol 28C

Status
Last updated on 6 April 2020, released on 16 April 2020
Organism
Saccharomyces cerevisiae S288C, Saccharomyces uvarum, mixed sample
Samples (12)
Protocols (6)
Description
Mitochondrial DNA (mtDNA) in budding yeast is biparentally inherited, but colonies rapidly lose one type of parental mtDNA, becoming homoplasmic. Therefore, hybrids between different yeast species possess two homologous nuclear genomes, but only one type of mitochondrial DNA. We hypothesise that the choice of mtDNA retention is influenced by its contribution to hybrid fitness in different environments, and that the allelic expression of the two nuclear sub-genomes is affected by the presence of different mtDNAs in hybrids. Here, we crossed Saccharomyces cerevisiae with S. uvarum under different environmental conditions and examined the plasticity of the retention of mtDNA in each hybrid.
Experiment types
RNA-seq of coding RNA, growth condition design
Contact
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-7922.idf.txt
Sample and data relationshipE-MTAB-7922.sdrf.txt
Links