E-MTAB-7878 - ATAC-seq of primary mammalian aortic endothelial cells before and after TNFα stimulation
Submitted on 29 July 2016, last updated on 6 April 2020, released on 1 June 2020
Bos taurus, Homo sapiens, Mus musculus
To identify conserved TNFα-induced changes in chromatin-accessibility in mammals, we performed ATAC-seq in primary vascular endothelial cells (ECs) isolated from the aortas of human (HAEC), mouse (MAEC) and cow (BAEC), before and after TNFα. We overlay our data with multi-species NF-κB binding data and identify multiple modes of NF-κB-chromatin interactions that are conserved during mammalian TNFα response. Our cross-species approach identifies conserved changes in chromatin-accessibility at NF-κB binding sites that are disease-relevant and essential during mammalian acute inflammation.
ATAC-seq, species design, stimulus or stress design