E-MTAB-740 - ChiP-seq of human breast cancer cell lines treated with estrogen and tamoxifen to investigate CTCF binding
Released on 7 December 2011, last updated on 3 June 2014
CCCTC-binding factor (CTCF) is a conserved zinc finger transcription factor involved in chromatin looping. Recent evidence has shown a role for CTCF in ER biology. This experiment maps CTCF binding genome-wide in breast cancer cells and shows that CTCF binding does not change with estrogen or tamoxifen treatment. We find a small but reproducible proportion of CTCF binding events that overlap with both the nuclear receptor estrogen receptor and the forkhead protein FoxA1. These overlapping binding events are likely to be functional as they are biased towards estrogen-regulated genes. In addition, we identify cell-line specific CTCF binding events. These cell-line specific CTCF binding events are more likely to be associated with cell-line specific ER vinding events and are also more likely to be adjacent to genes that are expressed in that particular cell line. These data suggest a positive, pro-transcriptional role for CTCF in ER-mediated gene expression in breast cancer cells.
ChIP-seq, cell type comparison, compound treatment
A co-ordinated interaction between CTCF and ER in breast cancer cells. Caryn S. Ross-Innes; Gordon D. Brown; Jason S. Carroll. BMC Genomics 12:593 (2011), PMID:22142239
A co-ordinated interaction between CTCF and ER in breast cancer cells. Ross-Innes CS, Brown GD, Carroll JS. :593 (2011), PMID:22142239