E-MTAB-7108 - Identification of genome wide Hoxa9 binding sites in primary murine and human AML cells
Submitted on 31 July 2018, last updated on 13 April 2019, released on 13 April 2019
Homo sapiens, Mus musculus
Relative overexpression of HOXA9 is a key feature of aggressive AML (acute myeloid leukemia). Here we determined genome wide binding sites of Hoxa9 in primary murine cells transformed by Hoxa9 and in a human AML cell line. In addition global H3K4 monomethylation and H3K27acetylation levels were determined in cells transformed by an inducible Hoxa9-ER construct in Hoxa9-active conditions and 72h after Hoxa9 was inactivated.
ChIP-seq, binding site identification design, time series design