E-MTAB-7107 - RNAseq of murine primary bone marrow cells transformed by an inducible Hoxa9-ER: time-course
Submitted on 31 July 2018, last updated on 13 April 2019, released on 13 April 2019
Relative overexpression of HOXA9 is a key feature of aggressive AML (acute myeloid leukemia). Hoxa9 responsive genes were identified by nascent RNA sequencing (4-thio-uridine labeled RNA - sequencing) in samples of primary hematopoietic precursor cells from mice transformed by an tamoxifen inducible version of Hoxa9 (Hoxa9-ER). Samples were generated in the presence of active Hoxa9 (0h) and in a time series after Hoxa9 was inactivated at 8h, 16h, 24h, 48h, and 72h.
RNA-seq of coding RNA, time series design