E-MTAB-7102 - Illumina microarray analysis of brain regions following acute exposure to bilirubin or LPS
Submitted on 22 August 2015, last updated on 3 August 2018, released on 26 August 2018
We developed a novel mouse model of acute bilirubin toxicity. 450mg/kg bilirubin was administered IP to mice, which exhibited transient hearing deficits and ataxia at 4 hours, and recovered by 24 hours. This toxicity is consistent with the human symptoms of bilirubin toxicity. To investigate the molecular mechanisms of bilirubin toxicity we carried out whole genome gene expression analysis on gross brain regions (cortex, cerebellum and brainstem) and specific parts of the auditory pathway (MNTB and CN) at 2 hours, 4 hours and 24 hours post injection. We detected several transcriptional markers of neuroinflammation, and so developed a second set of data for the same brain regions and time points following injection of a well characterized neuroinflammatory agent; LPS (5mg/kg).
transcription profiling by array, compound treatment design
Neuro-inflammation and ER-Stress are key mechanisms of acute bilirubin toxicity and hearing loss in a mouse model. Joshua L. Smalley, Emanuele Schiavon, Sherylanne Newton, Nigel H. Greig, Ian D. Forsythe.