E-MTAB-6960 - Microarray of five Caenorhabiditis elegans genotypes with or without a human alpha-synuclein transgene in the genetic background

Status
Last updated on 11 February 2019, released on 11 February 2019
Organism
Caenorhabditis elegans
Samples (32)
Array (1)
Protocols (5)
Description
Previous studies in Caenorhabditis elegans expressing the human alpha-synuclein protein, have shown that protein aggregation and some aetiologies of Parkinsons disease can be recapitulated in this model nematode. However, it is known that the genetic background is important in how the disease progresses in humans. This study sought to address this topic in the model nematode C. elegans. In this study we measured gene expression in C. elegans nematodes with different genetic backgrounds (JU511, JU1926, JU1931, and JU1941) with an introgression of and N2 region with the alpha-synuclein protein in chromosome IV. We measured expression in the wild-type strains and strains with the transgene. Each strain was measured in three biological replica's at an age of 48 hours post synchronization by bleaching.
Experiment types
transcription profiling by array, genotype design
Contact
Citation
Genetic background modifies phenotypic and transcriptional responses in a C. elegans model of α-synuclein toxicity. Wang YA, Snoek BL, Sterken MG, Riksen JAG, Stastna JJ, Kammenga JE, Harvey SC. 20(1):232 (2019), PMID:30894116
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-6960.idf.txt
Sample and data relationshipE-MTAB-6960.sdrf.txt
Raw data (2)E-MTAB-6960.raw.1.zip, E-MTAB-6960.raw.2.zip
Array designA-GEOD-10094.adf.txt