E-MTAB-6938 - Ribosome profiling of budding yeast Saccharomyces cerevisiae in eEF3 depleted conditions
Submitted on 10 June 2015, last updated on 29 June 2018, released on 30 June 2018
Ribosome profiling (Ribo-Seq) and RNA-Seq analysis of eEF3 depletion in yeast (Saccharomyces cerevisiae). eEF3 depletion was induced by methionine in a modified strain where the native promoter was replaced by methionine repressible MET25 promoter. Conditional depletion enables us to study global effects of an essential gene.
RNA-seq of coding RNA, translational bias design