E-MTAB-6868 - Human notochordal cell transcriptome in the developing intervertebral disc
Submitted on 21 October 2013, last updated on 29 August 2018, released on 29 August 2018
The adult nucleus pulposus originates from the embryonic notochord, but loss of notochordal cells with skeletal maturity in humans is believed to initiate intervertebral disc degeneration. Thus, defining the phenotype of human embryonic/fetal notochordal cells is essential for understanding their roles and for development of novel therapies. However, a detailed transcriptomic profiling of human notochordal cells has never been achieved. In this study, the notochord-specific marker CD24 was used to specifically label and isolate (using FACS) notochordal cells from human embryonic and fetal spines (7.5-14 weeks post-conception), which were studied by microarray analysis.
transcription profiling by array, cell type comparison design
Human notochordal cell transcriptome unveils potential regulators of cell function in the developing intervertebral disc. Rodrigues-Pinto R, Ward L, Humphreys M, Zeef LAH, Berry A, Hanley KP, Hanley N, Richardson SM, Hoyland JA. 8(1):12866 (2018), PMID:30150762