E-MTAB-6755 - RNA-seq analysis of Arabidopsis sulki1, Kas-2 and Ler/Kas-2 near isogenic line (NIL)
Submitted on 9 April 2018, last updated on 15 May 2018, released on 16 May 2018
Progeny from a cross of Arabidopsis thaliana Landsberg (Ler, Poland) and Kashmir-2 (Kas-2, central Asia) accessions exhibit immune-related hybrid incompatibility (HI) due to a genetic interaction between a cluster of eight TNL (Toll/Interleukin1 Receptor- Nucleotide Binding - Leucine Rich Repeat) RPP1-like (Recognition of Peronospora parasitica 1-like) genes (R1- R8) from Ler and central Asian alleles of a Strubbelig-family receptor-like kinase (SRF3). In characterizing mutants altered in Ler/Kas-2 HI, we mapped multiple intragenic mutations to the RPP1-like Ler locus. The expression of sulki1 (suppressor of Ler/Kas-2 incompatibility 1) mutant mapping to RPP1-like R8 Ler, the parental line Kas-2 accession and the Ler/Kas-2 NIL have been determined by RNA-seq in 5-week old plants grown at 14 - 16 ºC.
RNA-seq of coding RNA, case control design
NLR mutations suppressing immune hybrid incompatibility and their effects on disease resistance. Kostadin E. Atanasov, Changxin Liu, Alexander Erban, Joachim Kopka, Jane E. Parker and Rubén Alcázar.