E-MTAB-6706 - Expression profiling of K-Ras-driven lung carcinogenesis upon the genetic absence of Enpp2 from pulmonary cells
Submitted on 22 January 2017, last updated on 25 April 2018, released on 30 August 2018
To decipher the role of Enpp2 (ATX) in lung carcinogenesis, a replication-deficient adenovirus expressing the cre recombinase (Ad-Cre) was administered intratracheally to K-rasLSL-G12D/+Enpp2n/n mice, as well as to control K-rasLSL-G12D/+ littermates, thus removing the synthetic stop element (LSL; lox-stop-lox) and allowing the expression of the oncogene and the overtime development of LC, as well as the genetic deletion of enpp2 in the corresponding mice. RNA was extracted from 3 lung samples per genotype and hybriidized to Affy microarrays.
transcription profiling by array, genetic modification design