E-MTAB-6529 - RNA-seq analysis of HeLa cells treated with GapmeR antisense oligonucleotides
Submitted on 6 March 2017, last updated on 7 October 2018, released on 12 October 2018
The experiment was carried out to provide insights into biological function of a newly identified long noncoding RNA, PNCTR. HeLa cells were transfected with either a PNCTR-specific GapmeR antisense oligonucleotide or a non-targeting GapmeR control. Total RNAs were extracted at 24 hours post transfection, QC'd and hybridized with oligo(dT) magnetic beads to isolate the poly(A) RNA fraction. Stranded mRNA sequencing libraries were then prepared using the Illumina TruSeq Stranded mRNA Library Kit and sequenced using an Illumina HiSeq 2500 instrument.
RNA-seq of coding RNA, compound treatment design
A short tandem repeat-enriched RNA assembles a nuclear compartment to control alternative splicing and promote cell survival. Karen Yap, Svetlana Mukhina, Gen Zhang, Jason S. C. Tan, Hong Sheng Ong, and Eugene V. Makeyev. Molecular Cell :in press