E-MTAB-6281 - RNA-Seq data: RNA-IP (TAP-ZC3H30 pull down followed by RNA-Seq) and total transcriptomics (Effect of ZC3H30 gene deletion) in procyclic form of T. brucei

Status
Last updated on 6 December 2017, released on 16 December 2017
Organism
Trypanosoma brucei
Samples (12)
Protocols (14)
Description
The first 4 samples belong to the RNA-IP using in situ TAP tagged ZC3H30 in procyclic (insect) form of the parasite T. brucei Lister 427, 2 samples are Elu or eluate, and 2 are FL or flowthrough (unbound) sample. The other 8 samples are also from procyclic cells. 4 samples belong to DKO(ZC3H30 gene double knockout), 2 are non-stressed and 2 are heat shocked samples; the rest 4 samples are DKO-ectopic (ZC3H30 double knockouts, expressing, ectopic copy of ZC3H30) 2 are non-stressed and 2 are heat shocked samples. Heat Shock experiment was done at 39 degree Celsius.
Experiment types
RNA-seq of coding RNA, cell type comparison design
Contact
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-6281.idf.txt
Sample and data relationshipE-MTAB-6281.sdrf.txt
Links