E-MTAB-6273 - STAT1 and STAT3 ChIP-seq for naive and effector memory (Tem) CD4 T-cells after IL-6 treatment
Submitted on 15 February 2016, last updated on 8 February 2019, released on 8 February 2019
Naïve and activated T-cells has a different response to antigenic challenge. We examine whether a cytokine like IL-6 induces different responses through the Jak-STAT pathway to affect the functional characteristics of a given CD4 T‑cell subset. We isolated naïve and effector memory (Tem) CD4 T-cells to investigated STAT1 and STAT3 binding after 1-hour treatment with 20ng/ml IL-6 in the presence of anti-CD3/CD28.
ChIP-seq, binding site identification design