E-MTAB-6155 - RNAseq of mouse presomitic mesoderm segments along the anteroposterior embryo axis

Status
Submitted on 11 July 2017, released on 1 December 2017, last updated on 17 January 2018
Organism
Mus musculus
Samples (31)
Protocols (6)
Description
Somitogenesis is the segmentation of the developing embryonic body axis into somites and is guided by oscillating genes, which create waves of expression that travel across the presomitic mesoderm (PSM) from posterior to anterior. Upon arrival of a wave at the PSM's anterior end, a new somite is formed. To identify genes that are expressed in a wave-like pattern we dissected the PSM of four different mouse embryos (pre-turned), separated the left and right sides, and divided each into five segments, from posterior to anterior (sampling sites 1 to 5). Each segment was used to construct libraries for high-throughput RNA-sequencing. For one embryo, we also sequenced two somites.
Experiment types
RNA-seq of coding RNA, organism part comparison design
Contact
Citation
Defining murine organogenesis at single-cell resolution reveals a role for the leukotriene pathway in regulating blood progenitor formation. Ibarra-Soria X, Jawaid W, Pijuan-Sala B, Ladopoulos V, Scialdone A, Jorg DJ, Tyser RCV, Calero-Nieto FJ, Mulas C, Nichols J, Vallier L, Srinivas S, Simons BD, Gottgens B, Marioni JC.  (2018), PMID:29311656
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-6155.idf.txt
Sample and data relationshipE-MTAB-6155.sdrf.txt
Processed data (2)E-MTAB-6155.processed.1.zip, E-MTAB-6155.processed.2.zip
Links