E-MTAB-6066 - Single-cell RNA-seq of dermal fibroblasts stimulated with dsRNA (poly I:C) in a time course of 0, 4 and 8 hours
Last updated on 6 July 2018, released on 6 July 2018
Homo sapiens, Macaca mulatta, Mus musculus, Rattus norvegicus
Dermal fibroblasts from human, rhesus macaque, mouse and rat, stimulated with dsRNA (poly I:C) in a time course of 0,4 and 8 hours, profiled using the Smart-seq2 protocol.
The innate immune response - the expression programme that is initiated once a pathogen is sensed - is known to be variable among responding cells, as well as to rapidly evolve in the course of mammal evolution. To study the transcriptional divergence and cell-to-cell variability of this response, we stimulated dermal fibroblast cells from two primates (human and macaque) and two rodents (mouse and rat) with dsRNA - a mimic of viral RNA that elicits a rapid innate immune response. Subsequently, we profiled the response using bulk RNA-seq, scRNA-seq and ChIP-seq across the four species and across different time points.
RNA-seq of coding RNA from single cells, species design, stimulus or stress design, time series design