E-MTAB-5998 - Whole transcriptome analysis of rat peritoneal cells in a TGFβ1 induced mesothelial-to-mesenchymal transition

Status
Submitted on 24 August 2016, last updated on 18 June 2018, released on 18 June 2018
Organism
Rattus norvegicus
Samples (10)
Protocols (6)
Description
Peritoneal fibrosis is a major complication of long-term peritoneal dialysis (PD), leading to ultrafiltration failure and sometimes life threatening encapsulating peritoneal sclerosis. Fibrosis is driven by activated myofibroblasts that are derived, in part, from mesothelial-to-mesenchymal transition (MMT). We aimed to discover novel mediators of MMT and then experimentally exploit them to prevent peritoneal fibrosis. Using an antibody to HBME-1 and streptavidin nanobead technology, we first pioneered a novel method to purify rat mesothelial cells. After exposing mesothelial cells to transforming growth factor β1 (TGFβ1), we undertook RNAseq whole transcriptome analyses and outlined, the expression profile of sorted mesothelial cells at pre- and post- MMT.
Experiment type
RNA-seq of coding RNA 
Contact
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-5998.idf.txt
Sample and data relationshipE-MTAB-5998.sdrf.txt
Links