E-MTAB-5836 - Effect of stress on the binding affinity of yeast eIF4F components to their target RNAs
Submitted on 23 July 2012, last updated on 10 October 2017, released on 11 October 2017
Translation factors eIF4E and eIF4G form eIF4F, which binds to mRNAs to promote ribosome recruitment and translation initiation. We were interested in analysing the effects of stresses on eIF4F interactions with individual mRNAs. We used a RIP-seq approach to assess how mRNA associations with eIF4E, eIF4G1 and eIF4G2 change in response to three stresses: 1) addition of hydrogen peroxide; 2) amino acids withdrawal; and, 3) glucose withdrawal. We find that acute stress leads to changes in eIF4FmRNA interactions that are shared among each factor and across the stresses imposed.
RIP-seq, RNA-seq of coding RNA, growth condition design, replicate design, stimulus or stress design
Dynamic changes in eIF4F-mRNA interactions revealed by global analyses of environmental stress responses. Costello JL, Kershaw CJ, Castelli LM, Talavera D, Rowe W, Sims PFG, Ashe MP, Grant CM, Hubbard SJ, Pavitt GD. 18(1):201 (2017), PMID:29078784