E-MTAB-5818 - RNA-seq of mouse adipose stem and progenitor cells

Status
Submitted on 12 May 2017, last updated on 21 March 2018, released on 1 May 2018
Organism
Mus musculus
Samples (10)
Protocols (6)
Description
Population control for the scRNA-seq based analysis a well-established fraction of mouse subcutaneous adipose-derived stromal vascular fraction (SVF) cells that is generally considered to harbour adipogenic stem and progenitor cells (ASPCs). We collected Lin- (CD31- CD45- TER119-) CD29+ CD34+ SCA1+ cells from the mouse subcutaneous SVF of transgenic mice, in which red fluorescent protein (RFP) is induced in Dlk1-expressing cells upon feeding with tamoxifen. While CD29, CD34, and SCA1 are generally expected to enrich for stem cells, DLK1 has previously been suggested to specifically mark pre-adipocytes.
Experiment types
RNA-seq of coding RNA, cell type comparison design, species design, strain or line design
Contact
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-5818.idf.txt
Sample and data relationshipE-MTAB-5818.sdrf.txt
Processed data (11)Click to browse processed data
Links