E-MTAB-5756 - Detecting Early Signs of Heat and Drought Stress in Phoenix dactylifera (Date Palm)

Status
Last updated on 17 December 2017, released on 1 January 2018
Organism
Phoenix dactylifera
Samples (16)
Protocols (6)
Description
Phoenix dactylifera seedlings were exposed to heat, drought and combined heat & drought conditions in growth chambers. Leaf samples were collected for total RNA isolation (RNAseq, Illumina HiSeq 1000), and water soluble metabolites. The RNAseq of four biological replicates (two individuals per replicate) were compared against the control condition. Transcriptomics data suggests the combine heat and drought resembled heat response, whereas drought resembled more to control. The hallmarks of heat stress were visible in the transcriptomics data, such as protein misfolding, response to hydrogen peroxide and cell wall modification, as well as ABA signaling in the case of drought. Since the plants were exposed to the stress for several days before harvesting, the early signs of heat stress such as calcium and NO signaling were not detected anymore. In addition, data suggest a significant enrichment of circadian rhythm motifs in the differentially expressed genes in heat and combined heat and drought stresses, suggesting new stress avoidance strategies.
Experiment types
RNA-seq of coding RNA, case control design, growth chamber study, growth condition design
Contacts
Citation
Detecting Early Signs of Heat and Drought Stress in Phoenix dactylifera (Date Palm). Omid Safronov, Jurgen Kreuzwieser, Georg Haberer, Waltraud Schulze, Mohamed S. Alyousif, Naif Al-Harbi, Leila Arab, Peter Ache, Thomas Stempfl, Joerg Kruse, Klaus X. Mayer, Rainer Hedrich, Heinz Rennenberg, Jarkko Salojarvi, Jaakko Kangasjarvi. 12(6):e0177883 (2017), PMID:28570677
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-5756.idf.txt
Sample and data relationshipE-MTAB-5756.sdrf.txt
Links