E-MTAB-5561 - A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming
Submitted on 22 October 2015, released on 25 April 2017, last updated on 9 March 2018
In mice, the PIWI-piRNA pathway is essential to re-establish transposon silencing during male germline reprogramming. The cytoplasmic PIWI protein MILI mediates piRNA-guided transposon RNA cleavage as well as piRNA amplification. MIWI2-bound piRNAs and its nuclear localization are proposed to be dependent upon MILI function. Here, we demonstrate the existence of a piRNA biogenesis pathway that in the absence of MILI that sustains partial MIWI2 function and reprogramming activity.
Bisulfite-seq, all pairs, genetic modification design, genotyping design