E-MTAB-5339 - ChIP-seq of Escherichia coli RpoS during stationary phase
Submitted on 18 October 2012, last updated on 13 December 2016, released on 23 December 2016
Escherichia coli str. K-12 substr. MG1655
To determine sites where RpoS binds (and hence likely plays a direct role in transcription), we used ChIP-seq to map the association of RpoS across the Escherichia coli chromosome during stationary phase growth in minimal medium. To facilitate ChIP, RpoS was C-terminally SPA-tagged at its native locus.
ChIP-seq, binding site identification design