E-MTAB-5246 - Expression analysis of foetal and adult intestinal epithelial cells cultured in vitro
Submitted on 5 October 2013, last updated on 11 November 2016, released on 30 November 2017
Primary intestinal epithelial cells were isolated from the proximal part of the small intestine from either E16.5 foetal tissue or adult tissue and embedded in matrigel for culturing in in advanced F12/DMEM supplemented with EGF, R-spondin and Noggin. RNA was extracted from cultures established from independent animals and subjected to expression profiling.
transcription profiling by array, development or differentiation design