E-MTAB-5120 - Effects of digested onion extracts on intestinal gene expression using rat intestine slices

Status
Last updated on 26 September 2016, released on 29 September 2016
Organism
Rattus norvegicus
Samples (12)
Array (1)
Protocols (4)
Description
Rat small intestine precision cut slices were exposed for 6 hours to in vitro digested yellow (YOd) or white onion extracts (WOd) that was followed by transcriptomics analysis. The digestion was performed to mimic the digestion that in vivo takes place in the saliva, stomach and small intestine. A main question was to which extent the outcome of the biological interpretation of the transcription analysis (pathway analysis) depend on the model used. One outcome was that mainly processes related to oxidative stress, and especially the Keap1-Nrf2 pathway, were affected by onions in all three models.
Experiment types
transcription profiling by array, compound treatment design
Contact
Citation
Effects of Digested Onion Extracts on Intestinal Gene Expression: An Interspecies Comparison Using Different Intestine Models. Nicole J. W. de Wit, Marcel Hulst, Coen Govers, Jan van der Meulen, Angeline van Hoef, Geert Stoopen, Astrid Hamers, Arjan Hoekman, Ric de Vos, Toine F. H. Bovee, Mari Smits, Jurriaan J. Mes, Peter J. M. Hendriksen. , PMID:27631494
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-5120.idf.txt
Sample and data relationshipE-MTAB-5120.sdrf.txt
Raw data (1)E-MTAB-5120.raw.1.zip
Array designA-GEOD-11534.adf.txt
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