4 protocols
AccessionType
nucleic acid sequencing protocol
Libraries were sequenced on Illumina HiSeq2500 using TruSeq SBS Kit v3-HS (2x101, paired-end run).
nucleic acid library construction protocol
Fragmentation of 100 ng purified genomic DNA (gDNA) in 55 l Tris-EDTA buffer was done on Covaris S2. After library preparation from 100 ng of fragmented gDNA using Agilent SureSelectXT v5+UTR (75 Mb), libraries were purified, size validated and prepared for sequencing according to the manufacturer's protocols. Libraries were sequenced on Illumina HiSeq2500 using TruSeq SBS Kit v3-HS (2x101, paired-end run).
sample collection protocol
Cell lines were FACS-sorted and single cell clones were picked and phenotyped. Growth of sorted cell clones was observed over a four-week period by microscopy.
nucleic acid extraction protocol
Fragmentation of 100 ng purified genomic DNA (gDNA) in 55 l Tris-EDTA buffer was done on Covaris S2.