E-MTAB-491 - Transcription profiling by array of the brains of bees showing scout and non-scout behaviour
Released on 1 July 2011, last updated on 3 May 2014
Scouts and non-scouts (recruits) were collected by using a novelty-seeking� assay. Experiment was conducted in a large outdoor screened enclosure, which enabled us to exert complete control over the location and number of food resources while at the same time studying naturalistic honey bee foraging behavior. Foragers were first trained to a color-marked training feeder� that contained unscented 50% sucrose solution (m/v); this initially was the only food source available to them. After 2-3 days of training, a novel feeder� was set up in another location in the enclosure, with different color markings and an odor cue. The training feeder was maintained, providing the bees with two possible foraging locations, a familiar and a novel. Scouts were identified as bees that switched foraging from the training feeder to the novel feeder; only bees seen foraging at the novel feeder two or more times and at least once at the training feeder were collected as scouts. Non-scouts (recruits) were collected at the end of the experiments; these were bees that continued to forage at the training feeder, and were never observed to switch to the novel feeder.
transcription profiling by array, dye swap, innate behavior, loop, stimulated type
Brain Transcriptional Regulatory Network Quantitatively Predicts Behavior-Specific Gene Expression. Sriram Chandrasekaran, Seth A. Ament, James A. Eddy, Sandra Rodriguez-Zas, Bruce R. Schatz, Nathan D. Price, and Gene E. Robinson.
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