E-MTAB-4867 - Microarray analysis of abiotic stress responses in Arabidopsis thaliana rosette leaf tissue of Col-0 wild type plants and double/triple knockout mutants of aquaporins (pip2;1 pip2;2 and pip2;1 pip2;2 pip2;4)
Released on 20 June 2017, last updated on 11 July 2017
This experiment contains microarray measurements for 135 Arabidopsis thaliana rosette leaf samples covering three genotypes under six different environmental conditions. The three genotypes comprise the Col-0 wildtype and two loss-of-function mutants of aquaporins, a pip2;1 pip2;2 double mutant and a pip2;1 pip2;2 pip2;4 triple mutant (respective AGI locus identifiers: AT3G53420, AT2G37170, AT5G60660). The six conditions include control condition (well-watered, 22°C, 70% relative air humidity), drought stress (one week without watering), heat stress without changing the absolute humidity of the ambient air (6 hours at 33°C, 37% relative air humidity), heat stress with supplemented air humidity to maintain a constant vapor pressure deficit before and during the heat episode (6 hours at 33°C, 84% relative air humidity), and the combinations of drought pretreatment with each of the two heat stress variants (one week of drought followed by 6 hours of heat stress). Samples from all conditions were harvested at the same time (within 15 min starting at 5 p.m.).
transcription profiling by array, genotype design, stimulus or stress design
Relationships between drought, heat and air humidity responses revealed by transcriptome-metabolome co-analysis. Elisabeth Georgii, Ming Jin, Jin Zhao, Basem Kanawati, Philippe Schmitt-Kopplin, Andreas Albert, J. Barbro Winkler and Anton R. Schaffner. BMC Plant Biology (2017)