E-MTAB-4696 - Comparative genomic hybridization by array of rat brain- and spinal cord-derived microvascular endothelial cells, in basal and inflammatory conditions upon different time points of TNF-α-treatment or TWEAK-treatment or LPS-treatment
Submitted on 1 November 2009, last updated on 17 May 2016, released on 20 June 2016
The brain and spinal cord are endowed with particular vascular systems, known as the blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) respectively, which maintain homeostasis between nervous parenchyma and peripheral circulation. Despite these common features, the BSCB presents structural and functional differences resulting in distinct vulnerability to pathological insults when compared to the BBB. Although, the heterogeneity of endothelial cell types underlies their remarkable ability to sub-specialize and provide specific requirements for a given vascular bed, very little is known concerning intrinsic differences between microvascular endothelial cells (MECs) derived from brain (BMECs) and spinal cord (SCMECs), including their response to inflammation. We used Agilent Whole Rattus Genome Microarray 4X44K to compare rat BMECs and SCMECs in both basal and inflammatory conditions; TNF-α-induced, TWEAK-induced and LPS-induced gene expression after 6 hr, 12 hr, 24 hr and 48 hr incubation.
comparative genomic hybridization by array, cell type comparison design, compound treatment design, stimulus or stress design, validation by real time PCR design