E-MTAB-4462 - DNA methylation profiling of human naive embryonic stem cells

Status
Released on 1 March 2016, last updated on 11 June 2019
Organism
Homo sapiens
Samples (12)
Protocols (6)
Description
Human pluripotent cell lines were derived from blastocyst-stage embryos and propagated in self-renewal conditions that maintain features of naive pluripotency characteristic of mouse embryonic stem cells. Genome-wide DNA methylation status of HNES1 and HNES3 naive and primed cells was assessed with post-bisulfite adapter tagging (PBAT).
Experiment types
methylation profiling by high throughput sequencing, cell type comparison design, growth condition design, organism part comparison design
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Citation
Naive pluripotent stem cells derived directly from isolated cells of the human inner cell mass. Ge Guo, Ferdinand von Meyenn, Fatima Santos, Yaoyao Chen, Wolf Reik, Paul Bertone, Austin Smith, Jennifer Nichols. Stem Cell Reports 6(4):437-446 (2016), PMID:26947977
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
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