E-MTAB-4427 - Transcription profiling by high throughput sequencing of rice under inorganic phosphate limiting conditions
Released on 25 November 2013, last updated on 2 February 2016
Oryza sativa Japonica Group
To better understand the complex mechanisms regulating Pi homeostasis in rice (Oryza sativa L. cv. Nipponbare), a time course experiment was performed, where pre-germinated seedlings were grown hydroponically for two weeks on Pi-sufficient medium (0.32 mM Pi), before transferring half of the plants to Pi-deficient solution (0 mM Pi) for 21 days (d). After three weeks of Pi-starvation treatment, half of these plants where then re-supplied with Pi sufficient media for up to 24 hours (h). In total, nine time points were selected in order to cover short and long term responses to Pi starvation as well as the effects of Pi re-supply on Pi starved plants.
other, RNA-seq of coding RNA
Spatio-temporal transcript profiling of rice roots and shoots in response to phosphate starvation and recovery. Secco D, Jabnoune M, Walker H, Shou H, Wu P, Poirier Y, Whelan J. , PMID:24249833