E-MTAB-4348 - Transcription profiling by high throughput sequencing of rice grain from from heat-tolerant and -sensitive lines in response to high night temperatures at the early milky stage
Released on 1 December 2014, last updated on 5 February 2016
Oryza sativa, seed metagenome
We analyzed the transcriptome profiles for rice grain from heat-tolerant and -sensitive lines in response to high night temperatures at the early milky stage using the Illumina Sequencing method. On the 8th day after the labeled florets flowered, plants with the same label were transferred to chambers and maintained at a temperature of 38.0 ± 0.5°C (treatment) or 25.0 ± 0.5°C (control) for the dark period (10 h), and 26.0 ± 0.5°C (both treatment and control) for the light period (14 h). Three biological replicates of the temperature treatments were grown under the same conditions. After 48 h of treatment, samples containing 45 grains with labels from the same region (middle to bottom part) of labelled ears were harvested, packed in aluminum foil, and flash-frozen in liquid nitrogen until further use. A total of 12 rice grain samples were harvested, i.e., controls (TC1, TC2 and TC3) and treatments (TT1, TT2 and TT3) of the three biological replicates of the heat-tolerant line, and controls (SC1, SC2 and SC3) and treatments (ST1, ST2 and ST3) of the three biological replicates of the heat-sensitive line.
RNA-seq of coding RNA
Transcriptome changes in rice (Oryza sativa L.) in response to high night temperature stress at the early milky stage. Liao JL, Zhou HW, Peng Q, Zhong PA, Zhang HY, He C, Huang YJ. , PMID:25928563