E-MTAB-4038 - Genome-wide profiling of Alternative polyadenylation sites in mouse female germline stem cells
Last updated on 10 November 2015, released on 20 November 2015
Alternative polyadenylation (APA) is an important post-transcriptional modification implicated in development. Female germline stem cell (FGSC) is unipotent and capable of giving rise to oocyte. However, whether alternative polyadenylation plays a role in self-renew and cell fate determination of FGSCs remain elusive. Here, we used 3T-Seq developed in our lab to profile genome-wide 3a termini of transcripts and delineate APA sites in mouse FGSCs and explored the biological significance of APA modulation in FGSC identity.
RNA-seq of coding RNA, cell type comparison design, development or differentiation design