E-MTAB-4038 - Genome-wide profiling of Alternative polyadenylation sites in mouse female germline stem cells

Status
Last updated on 10 November 2015, released on 20 November 2015
Organism
Mus musculus
Samples (2)
Protocols (5)
Description
Alternative polyadenylation (APA) is an important post-transcriptional modification implicated in development. Female germline stem cell (FGSC) is unipotent and capable of giving rise to oocyte. However, whether alternative polyadenylation plays a role in self-renew and cell fate determination of FGSCs remain elusive. Here, we used 3T-Seq developed in our lab to profile genome-wide 3a termini of transcripts and delineate APA sites in mouse FGSCs and explored the biological significance of APA modulation in FGSC identity.
Experiment types
RNA-seq of coding RNA, cell type comparison design, development or differentiation design
Contact
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-4038.idf.txt
Sample and data relationshipE-MTAB-4038.sdrf.txt
Links