E-MTAB-3887 - Bicyclus anynana RNA-Seq raw sequencing reads
Submitted on 9 September 2015, last updated on 21 September 2015, released on 1 October 2015
Bicyclus anynana butterflies were reared at 17°C and 27°C to produce the dry and wet season forms. RNA was extracted using TRIzol from the heads of 12 individual animals ~0-3 hours after eclosing; 3 dry season females, 3 wet season females, 3 dry season males, and 3 wet season males. A TruSeq RNA Sample Preparation Kit v2 was used to make 12 double stranded cDNA libraries from polyadenylated RNA. We size selected for DNA at ~280-340 bp. Libraries were sequenced using a HiSeq 2500, paired end 100-cycle sequence run.
RNA-seq of coding RNA
Transcriptome-wide differential gene expression in Bicyclus anynana butterflies: Female vision-related genes are more plastic. Aide Macias-Munoz, Gilbert Smith, Antonia Monteiro and Adriana D. Briscoe.