E-MTAB-3863 - Identification of genes enriched in nociceptive neurons of Drosophila larvae

Status
Submitted on 1 July 2008, last updated on 2 January 2016, released on 6 March 2016
Organism
Drosophila melanogaster
Samples (8)
Array (1)
Protocols (5)
Description
This study aims to identify genes whose expression is enriched in the nociceptive neurons of Drosophila larvae. These neurons are involved in detecting noxious mechanical and noxious thermal stimuli. Four replicates each for Class I md-da neurons (2-21Gal4>>UAS-mCD8GFP) and Class IV md-da (ppk-GAL4>>UAS-mCD8GFP) neurons were analyzed. RNA from 50-60 neurons for each replicate was isolated by laser capture microdissection. RNA was amplified using the WT-Ovation Pico Kit from NuGen technologies. RNA was fragmented and labeled using the FL-Ovation cDNA Biotin Module V2 (NuGen Technologies). RNA was hybridized to arrays and data scanned at the Translational Genomics Research Institute.
Experiment types
transcription profiling by array, cell type comparison design, organism part comparison design, replicate design
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-3863.idf.txt
Sample and data relationshipE-MTAB-3863.sdrf.txt
Processed data (1)E-MTAB-3863.processed.1.zip
Array designA-AFFY-35.adf.txt
Links